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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
Anti Histamine H1 Receptor Hrh1 Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
Anti Histamine H2 Receptor Hrh2 Rabbit Polyclonal, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
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Alomone Labs rabbit polyclonal anti ox1r 488
<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
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<t>P2X1</t> receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals
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Image Search Results


P2X1 receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals

Journal: Purinergic Signalling

Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea

doi: 10.1007/s11302-026-10129-7

Figure Lengend Snippet: P2X1 receptor expression in the adult Wistar rat cochlea. Cryosections ( A - B , E ) and whole mount ( C - D , F ) from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin ( green ) and anti-P2X1 antibody ( red ), and counterstained with DAPI ( blue ). (A-B) Representative image showing the overviews of rat cochlear cryosections ( A ) and the region with the organ of Corti and Rosenthal’s canal ( B ) showing P2X1 receptor expression in the organ of Corti (OoC) and the spiral ganglion neurons (SGN). (C-D) 6–8-week-old rat cochlea whole mount OoC preparation showing P2X1 immunolabelling, focusing on the SGN processes underneath the inner and outer hair cells ( C ) and the corresponding region in P2X1 antibody peptide block control ( D ). ( E -E”) Rosenthal’s canal of cryosection from ( B ). ( F -F’) 6–8-week-old OoC rat cochlea whole mount z-stack images showing tunnel crossing fibres labelled with anti-P2X1 antibody (arrowheads) and P2X1 immunolabelling at the base of the OoC (*). Scale bars, 50 μ m (A), 40 μ m ( B - E ), 20 μ m ( F ). Representative images from n = 4 cochleae from four different animals

Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution).

Techniques: Expressing, Blocking Assay, Control

Co-expression of P2X1 receptors and peripherin-1 in Wistar rat cochlea. ( A - C ) Cryosections from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin (green) and anti-P2X1 antibody (red), and counterstained with DAPI (blue). ( F ) White arrows indicate SGNs with strong P2X1 signals relative to other SGNs. Images were taken of the basal ( A ), mid-( B ) and apical ( C ) regions of the cochlea. Scale bar = 20 μm for ( A - C ). ( D - G ) Cryosections from 6–8-week-old Wistar rat cochlea ( D - E ) and P8 cochlea ( F - G ) were co-labelled with anti-peripherin-1 antibody ( green ), and anti-P2X1 antibody ( red ). Images show the overview from the apical turn ( F ) and spiral ganglion neurons in the apical region ( G -G”). Representative images from n = 4 cochleae from four different animals

Journal: Purinergic Signalling

Article Title: Expression of the P2X1 receptor remains in the type II spiral ganglion neurons in the mature rat cochlea

doi: 10.1007/s11302-026-10129-7

Figure Lengend Snippet: Co-expression of P2X1 receptors and peripherin-1 in Wistar rat cochlea. ( A - C ) Cryosections from adult (6–8 week-old) Wistar rat cochleae were immunolabelled with anti-βIII tubulin (green) and anti-P2X1 antibody (red), and counterstained with DAPI (blue). ( F ) White arrows indicate SGNs with strong P2X1 signals relative to other SGNs. Images were taken of the basal ( A ), mid-( B ) and apical ( C ) regions of the cochlea. Scale bar = 20 μm for ( A - C ). ( D - G ) Cryosections from 6–8-week-old Wistar rat cochlea ( D - E ) and P8 cochlea ( F - G ) were co-labelled with anti-peripherin-1 antibody ( green ), and anti-P2X1 antibody ( red ). Images show the overview from the apical turn ( F ) and spiral ganglion neurons in the apical region ( G -G”). Representative images from n = 4 cochleae from four different animals

Article Snippet: The primary antibodies used were: rabbit polyclonal anti-P2X1 antibody (Alomone Labs, Jerusalem, Israel, Catalog# APR-001, 1:500 dilution), mouse anti-β III tubulin (IgG2a monoclonal, BioLegend, San Diego, CA, catalog No. 801213, 1:1000 dilution), goat polyclonal anti-peripherin-1 antibody (Everest Biotech, Upper Heyford, UK, catalog No. EB12405, 1:1000 dilution).

Techniques: Expressing